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Image Search Results
Journal: Journal of pharmacological sciences
Article Title: Compensatory role of neuregulin-1 in diabetic cardiomyopathy.
doi: 10.1016/j.jphs.2023.08.009
Figure Lengend Snippet: Fig. 4. Klf10 was upregulated in the ventricles of STZ mice, A qRT-PCR analysis of Klf4 in the ventricle of control, STZ-4W or insulin-treated STZ-4W STZ-4W þ Ins) mice. Each data represents the mean ± SEM (n ¼ 6e9). ****p < 0.0001 by ANOVA post hoc Tukey's test. B. The correlation between the expression levels of Klf4 and Klf10 (r ¼ 0.3989, p ¼ 0.0733). C qRT-PCR analysis of Klf10. Each data represents the mean ± SEM (n ¼ 6e9). *p < 0.05 by ANOVA post hoc Tukey's test. D. The correlation between the expression levels of Nrg1 and Klf10 (r ¼ 0.7935, p ¼ 0.0001). E. Perinuclear localization of Klf 10 (green), CD31 (magenta) and DAPI (cyan) in the ventricles of control and STZ-4W mice. For RT-PCR, relative quantification (RQ) of the gene expression was calculated with the comparative Ct method. Hprt was used as an internal reference.
Article Snippet: Cryostat sections (15 mm) were incubated overnight at 4 C with the following primary antibodies: mouse antieneuregulin-1 monoclonal antibody (1:100; Santa Cruz Biotechnology, Dallas, TX, USA), rat anti-CD31 monoclonal antibody (1:50; BD Pharmingen, Franklin Lakes, NJ, USA), or
Techniques: Quantitative RT-PCR, Control, Expressing, Reverse Transcription Polymerase Chain Reaction, Gene Expression
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats
doi: 10.1155/2016/9651592
Figure Lengend Snippet: Primers for real-time PCR.
Article Snippet: Nonspecific binding sites were blocked with 5% milk powder diluted in TBS with 0.05% Tween 20 (TBST) for 60 min. Proteins were detected using the following antibodies: rabbit polyclonal antibody for PI3K-p85 (diluted 1 : 3000; Bios; bs-0128R), rabbit polyclonal antibody for phospho-PKC ζ / λ (diluted 1 : 3000; CST; #9378),
Techniques:
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats
doi: 10.1155/2016/9651592
Figure Lengend Snippet: Effects of acupuncture on mRNA expressions of IRS1, IRS2, PI3K-p85 α , Akt2, aPKC ζ , aPKC λ , and GLUT4 in skeletal muscle of OLETF rats. Acupuncture was applied to the indicated region for 20 min per day, over the course of 21 days. Following administration, the musculus quadriceps femoris was obtained. Relative mRNA expression was determined by real-time RT-PCR. Data are showed as mean ± standard deviation (SD) ( n = 8 each group). ∗∗ P < 0.01 versus SD group; △ P < 0.05 and △△ P < 0.01 versus OLETF group. IRS1: insulin receptor substrate 1; IRS2: insulin receptor substrate 2; PI3K: phosphatidylinositol-3 kinase; PKC: protein kinase C; GLUT4: glucose transporter 4; Akt2: protein kinase B beta.
Article Snippet: Nonspecific binding sites were blocked with 5% milk powder diluted in TBS with 0.05% Tween 20 (TBST) for 60 min. Proteins were detected using the following antibodies: rabbit polyclonal antibody for PI3K-p85 (diluted 1 : 3000; Bios; bs-0128R), rabbit polyclonal antibody for phospho-PKC ζ / λ (diluted 1 : 3000; CST; #9378),
Techniques: Expressing, Quantitative RT-PCR, Standard Deviation
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats
doi: 10.1155/2016/9651592
Figure Lengend Snippet: Effects of acupuncture on protein expressions of PI3K-p85, phospho-PKC ζ / λ , and GLUT4 in skeletal muscle of OLETF rats. Protein expression was determined by Western blot. GAPDH was used as an internal control. Data are shown as mean ± SD ( n = 8 each group). ∗ P < 0.05 and ∗∗ P < 0.01 versus SD group; △△ P < 0.01 versus OLETF group.
Article Snippet: Nonspecific binding sites were blocked with 5% milk powder diluted in TBS with 0.05% Tween 20 (TBST) for 60 min. Proteins were detected using the following antibodies: rabbit polyclonal antibody for PI3K-p85 (diluted 1 : 3000; Bios; bs-0128R), rabbit polyclonal antibody for phospho-PKC ζ / λ (diluted 1 : 3000; CST; #9378),
Techniques: Expressing, Western Blot, Control